Problem:
You have discovered a novel organism that thrives in the ocean depths in the hostile environment of hydrothermal vents. In characterizing its replication, you are astounded to find that it replicates both strands of DNA continuously, using two DNA polymerases; one that synthesizes in the usual 5'-to-3' directions and one that synthesizes in the 3'-to-5' direction. Both polymerases use the standard nucleoside 5'-triphosphates for addition of nucleotides to the growing chain. You are surprised to find that both newly synthesized DNA strands are made with the same high degree of fidelity that characterizes replication in E. coli.
Required:
Question 1: Briefly explain three processes that contribute to the high fidelity of DNA replication in E. coli.
Question 2: Explain why it is surprising that both strands of DNA in this novel organism are replicated with high fidelity.
Question 3: Suggest a way by which high fidelity might be accomplished in 3'-to-5' replication. If you invent enzymes as a part of your answer you must explain how they work.
Please can anyone help me to answer these question in detail.