Question: We have studied two methods to inhibit TORC2 in this course, one being Palmitoylcarnitine, the other being rapamycin treatment to cells expressing TOR1-1 and a TORC2 complex made up of a truncated Avo3. What are the differences? Palmitoylcarnitine inhibits TORC2 in vitro, but rapamycin only inhibits TORC2 (containing the Avo3 truncation) in cells One inhibitor functions through a direct interaction, the other is indirect One molecule forms a ternary complex with TORC2 and FKBP12, the other does not Palmitoylcarnitine causes a decrease in membrane tension: inhibition of TORC2 with rapamycin causes an increase in membrane tension Neither method works on wild type cells. Need Assignment Help?