Problem:
Question 1: Using a regular light microscope what is the main difference you expect to observe when viewing cultures of macrophages infected with amastigotes of either Leishmania donovanior Leishmania mexicana.
Question 2: If you re-examined these cells using a fluorescent microscope and appropriate fluorochrome-labeled antibodies, identify three markers that characterise the parasite-containing vacuole.
Question 3: Outline a simple experiment to study the fusogenic potential of the Leishmania containing vacuole with other intracellular compartments.
Could someone help to explain the answer of these questions.