Problem:
The principle pathway for transport of lysosomal hydrolases from the trans Golgi (normally pH 6.6) to the late endosomes (normally pH 6) and for recycling of mannose- 6-phosphate (M6P) receptors back to the Golgi depends on the pH difference between those two compartments. From what you know about M6P receptor binding and recycling and the pathways for delivery of material to lysosomes, describe the consequences of changing the pH in those two compartments.
Required:
Question 1: What do you suppose would happen to M6P receptor binding and recycling if the pH in late endosomes were raised to pH 6.6?
Question 2: What do you suppose would happen to M6P receptor binding and recycling if the pH in the trans Golgi network were lowered to pH 6?
Could anyone help me please?