Problem
A. What is required for RNA polymerase to synthesize DNA? How is this different from what is required by DNA polymerase?
B. What are the two mechanisms for transcription termination in bacteria-and how do they work?
C. How is transcription by RNA polymerase II (eukaryotes) catalyzed?
D. Indicate the components of the bacterial RNA polymerase core and holoenzyme.
a. What is the role of sigma factor?
E. Indicate the RNA polymerases in eukaryotes and what they primarily transcribe.
F. Define the template and coding strands of DNA.
G. Indicate the major steps in transcription (bacteria) and what happens at each step.
H. How do actinomycin D, rifampicin and amanitin block transcription? Indicate if they are effective against bacteria, eukaryotes or both.
I. What is the structure of promoters for RNA polymerase I, II and III of eukaryotes?
J. What are kinetic and nucleolytic proofreading and how are they used?