Define some Precautions for Estimation of the Amount of Bacteria?
1. Dilution should be made carefully.
2. Use fresh sterile pipette for making each dilution.
3. Aseptic condition should be made.
4. Proper mixing of nutrient agar with the bacterial culture should be made for even distribution of cells.
5. Plates containing colonies between 30 and 300 should be used for microbial counting.
6. Count all surface and subsurface colonies.