Define Regents for Determination of Inorganic Phosphorus?
1. Trichloroacetic acid (10%) - Dissolve 100 g of high grade crystalline acid in distilled water and dilute to 1 Litre.
2. 10 N Sulphuric acid - Slowly add 450 ml of concentrated sulphuric acid to 1300 ml distilled water in a pyrex container. Allow to cool. Store in a glass stoppered pyrex bottle.
3. Ammonium molybdate solution 2.5% in 3 N sulphuric acid. Dissolve 12.5 g of ammonium molybdate in I00 ml distilled water in a 500 ml volumetric flask. Add 160 ml of 10 N sulphuric acid solution and dilute to 500 ml with distilled water.
4. 15% sodium bisulphite solution - Add 30 g of reagent grade sodium bisulphate in distilled water and dilute to 200 ml. Use only clear supernatant.
5. 20% sodium sulphite solution - Dissolve 20 g of reagent grade anhydrous sodium sulphite in distilled water and dilute to 100 ml. Filter if necessary and keep in a well stoppered flask.
6. Amino napthol sulphonic acid solution (ANSA) - Place 195 ml of the sodium bisulphate solution in a suitable glass stoppered container and add 0.5 g of 1,2,4 amino napthol sulphonic acid and 5 ml of sodium sulphite solution. Stopper and shake until dissolved. If it is not completely dissolved add more of sulphite, 1 ml at a time till completely dissolved. Pour in a dark glass bottle and store in a refrigerator.