Define Precaution for Separation of Amino Acids by Paper Chromatography?
1. Hold the paper with the extra strip kept at one side. Handling of whatman paper with hand should be avoided.
2. The spotting of amino acid should not be more than 1 mm in diameter since large spots lead to diffused and large amino acid patterns with no sharp outline.
3. The petridish should be saturated with solvent vapours.
4. Solvent front should be marked immediately after removing teh chromatogram, since it disappears rapidly.
5. The outline of each amino acid should be immediately marked with pencil, Do not mark with ball point or ink pen.