Discussion:
Microbiology Questions on competency, lysogeny & dilution
1. Define natural competency?
2. What is a serial dilution?
3 True or False. You can use plaque assay to determine the number of a lysogenic phage?
4. Can a spectrophotometer be used to measure viable counts of bacteria in culture?
5. Is transmittance or absorbance directly related to the concentration particles present in a solution?
6. Add 1 ml sample to 9 ml diluent → 1:10 (1/10, or 10-1or -1) dilution
Add 1 ml sample to 99ml diluent → 1:100(1/100, or10-2 or -2) dilution
Add 0.1ml to 0.9 ml diluent → ? dilution
(use the 1st two examples as a guide to do the 3rd one).