1. Watson and Crick revealed the structure of DNA in 1953. However the description of DNA began 135 years prior by a man named Friedrich Miescher (Dahm,2005). Briefly describe this first experiment. What cells were used, what was his original focus, since it was not DNA? What type of cells did he eventually work with and what did he almost determine?
2. Define In vivo and Invitro (include a reference).
3. Is cloning an In vivo or In vitro process? Explain?.
4. The recombinant plasmid was isolated from what organism (no abbreviations genus and species)?
5. True or False. The isolated recombinant plasmid is part of the genome of the organism. Explain.
6. Ligation and Clonging- define vector, insert, and host cell (include a reference).