Four Hfr stains of E. coli are known to transfer their genetic material during conjugation in different sequences. Given the time of entry of the markers into the F- recipient:
A. Construct a genetic map that includes all of these markers. (12 points)
B. Label the time distance between adjacent gene pairs. (11 points)
C. Indicate the points at which the F factor is located for each of the strains. (4 points)
Hfr Strain 1 Markers arg thy met thr - -
Times in min 15 21 32 48 - -
Hfr Strain 2 Markers mal met thi thr try -
Times in min: 10 17 22 33 57 -
Hfr Strain 3 Markers phe his bio azi thr thi
Times in min 6 11 33 48 49 60
Hfr Strain 4 Markers his phe arg mal - -
Times in min 18 23 35 45 - -