1.) How is PCR superior to colony hybridization for identifying DNA sequences of interest?
2.) What is the natural function of bacterial restriction endonucleases? How might the presence of restriction enzymes affect the use of bacteria as hosts for carrying cloned genes?
3.) Gel electrophoresis analysis of a PCR reaction generated with a pair of oligos showed three fragments instead of the expected single fragment. Give possible explanations for the result.
4.) How would you clone the metabolic genes involved in trp biosynthesis from ecoli?