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Clculate the kcat in bull sec-1 and catalytic efficiency

From a kinetics experiment, the V_max was determined to be 3200111\4.min^-1. For the kinetic assay, 0.2mL, of a 0.05mg/mL solution of enzyme was used, and the enzyme has a molecular weight of 95,000 g/mole.

Assume a reaction volume of 3mL.

Calculate the k_cat (in • sec^-1) and catalytic efficiency ( in M^-1sec-¹) for the enzyme.

The Km for the enzyme was determined to be 2.5 x 10^-2M.

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Chemistry: Clculate the kcat in bull sec-1 and catalytic efficiency

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