Chemicals in the rna isolation procedure, Biology

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Chemicals in the rna isolation procedure

What are the reasons for using the following chemicals in the RNA isolation procedure?
-Monophasic lysis solution
-70% ethanol
-Liquid Nitrogen
-LiCl or other salts

-How will you set up a restriction digestion in 20 ul reaction containing 2 ug genomic DNA (concentration 200 ng/ul) and 5 units of EcoRl enzyme/ug DNA (10 units/ul) and 1 x reation buffer (stock 10 X)? Show all calculations.

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