Discussion:
Catalytic Mechanism of Carboxypeptidase A
1) Carboxypeptidase A, a zinc-containing enzyme, hydrolyzes the C- terminal peptide bonds of polypeptides and/or proteins. In the enzyme- substrate complex, the zinc ion is coordinated to three enzyme side chains, the carbonyl oxygen of the scissile peptide bond, and a water molecule. A plausible model for the enzyme's reaction mechanism that is consistent with X-ray and enzymological data is diagrammed below.
Attachment:- Practice problem.rar