Calculate the expected cell density obtained after four days, Biology

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Calculate the expected cell density obtained after four days

Problem

A 5 ml cell suspension of density 8.5 x 10⁷ cells/ml was completely sub- cultured with a split-ratio of 1:6 into T75 flasks. Calculate the expected cell density (in cells/cm²) obtained after 4 days of growth, given that the doubling time of the cell line is 20 hours and assuming that the cells remain in exponential phase. Show your working.

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