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Assignment tem calibration- what might be a good method for

Assignment: TEM Calibration

1. What might be a good method for magnification calibration at even higher magnifications (>195000)?

2. Why does the diffraction standard specimen generate a ring pattern rather than a spot pattern?

3. How do you index the diffraction rings in the above diffraction ring pattern?

4. Why may there be a rotation angle between the image and diffraction pattern in a TEM?

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