An adsorption chromatographic separation of a mixture of, Mechanical Engineering

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An adsorption chromatographic separation of a mixture of

Although temperature does not have nearly the effect on HPLC separations that it has on GC separations, it nonetheless can play an important role. Discuss how and why temperature might or might not influence the following separations:

(a) A reversed-phase chromatographic separation of a steroid mixture.

(b) An adsorption chromatographic separation of a mixture of closely related isomers.

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