Aliquots of a 05-mgml standard of bsa are used to construct


Aliquots of a 0.5-mg/mL standard of BSA are used to construct a standard curve for the Bradford protein assay. The tubes contain the following amounts of the BSA solution: 0, 20, 40, 60, 80, and 100 µL. The corresponding absorbencies after adding Bradford reagent are 0, 0.05, 0.09, 0.14, 0.19, and 0.22. If you take 20 µL of an unknown and add 80 µL of water, mix, take 10 µL of the mixture and add to Bradford reagent, and see an absorbance of 0.08, what is the protein concentration of the undiluted unknown?

Request for Solution File

Ask an Expert for Answer!!
Chemistry: Aliquots of a 05-mgml standard of bsa are used to construct
Reference No:- TGS0779531

Expected delivery within 24 Hours