After you have added 1 n hcl to adjust the ph of the tris
Use Henderson-Hassalbach equation. The pKa of Tris-HCl is 8.1. After you have added 1 N HCl to adjust the pH of the Tris solution to 8.1, what is the ratio (in mmols) of Tris to Tris-HCl in the resulting buffer solution?
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After you have added 1 N HCl to adjust the pH of the Tris solution to 8.1, what is the ratio (in mmols) of Tris to Tris-HCl in the resulting buffer solution
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Based on the Henderson-Hassalbach equation how many mmols of Tris-HCl are there in a 100 mL of a 100 mM Tris-HCl buffer solution at pH 8.1? Note that the 100 mM refers to the sum of Tris and Tris-HCl concentrations.
Remember that M1V1=M2V2. What volume of distilled water do you need to add to dilute 10 mL of a 100 mM Tris- HCl buffer solution to a concentration of 10 mM
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