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After a pcr run using the new set of primers you perform

You have been successfully performing PCR using primers to amplify a specific gene. However, after several weeks and a dozen successful PCR reactions, you have depleted your primer stock. After a PCR run using the new set of primers, you perform electrophoresis to evaluate your PCR products; however, your gel shows no amplification bands at all. Suggest three reasons WITH explanations that could lead to such a result?

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