Discussion:
Absorption Spectra for Fluorescent Proteins
1. What is the difference between an absorption spectrum, excitation spectrum, and emissions spectrum?
2. I deduce the identity of two fluorescent protein (label FP3 and FP6) based on absorption spectrum.
3. The results of the absorption spectra were plotted in two ways: Y-axis as true absorbance or Y-axis as normalized absorbance. How do the two spectra differ? What information is lost by plotting the Y-axis as normalized absorbance and not true absorbance.
4. Please help me understand the absorption spectra that I got for my two fluorescent protein.
Please see attached for more detail
Attachment:- absorption spectra.rar