A U-shaped tube with a fine filter separating the right and left arms is filled with complete media. Liquid may pass between the arms of the tube, but the filter is just fine enough to prevent any cells from passing through. On the right is a leu- arg- strain of E. coli. Fill in the blanks
a) The media contains DNAse. A leu+ arg+ strain of E. coli is added to left side. After the experiment you find that some colonies form when the right strain is grown on media without leucine and arginine. ………….. between cells on the …………. side to cells on the …………… side had to occur.
b) The media contains no DNAse. Cell lysate from the leu+ arg+ strain is added to the left side. After the experiment you find that some colonies form when the right strain is grown on media without leucine and arginine. Chromosomal DNA from the …………….. side ……………. cells in the ……………….. side.
c) ………………… will never happen between the two strains in the left and right tubes because …………………….