A student performed thin layer chromatography as explained in this experiment but made the following mistakes or changes in the procedure. In each case, define how the change/mistake would affect the Rf value:
A. student failed to close the container containing the developing solvent and in which the thin layer chromatography run was carried out
B. the solvent level in the developing jar was above the origin line on the thin layer chromatography sheet
C. student utilized as developing solvent, a mixture of two immiscible solvents
D. student spotted too much of a concentrated sample of the compound to be chromatographed
E. the student dozed off as well as the solvent front ran off the thin layer chromatography plate before he stopped the run