A formate buffer (pka = 3.75) is prepared by mixing 50 mL of a 4 M formic acid and 2 g of solid sodium hydroxide (MW= 40g/mol) in total volume of 1.0 L. A peptide with the amino acid sequence C-P=T-Y-R-L-D-Q (MW = 995.12 g/mol) is dissolved in this solution at a concentration of 5 micromol.
a. What is the pH of this solution?
b. What is the net charge of the peptide in solution?
c. Calculate the approximate pH at which this peptide would have a net charge of zero.