A micro channel 10 µm tall, 50 µm wide, and 2 cm long is used for analysis of bradykinin, a small peptide with a molecular weight of 1.06 kDa (1 Da = 1 g/mol).
a. If the channel is filled with a 1 ng/mL solution of bradykinin, approximately how many molecules are in the channel?
b. What is the morality of the bradykinin sample (i.e. convert µg/mL to M)?
c. The micro fluidic substrate is fabricated from two 500 µm thick glass plates bonded together. An inlet reservoir 2 mm in diameter is drilled through one of the plates to provide fluidic access to one end of the micro channel. Assuming the reservoir is completely filled with bradykinin sample, how many molecules are in the reservoir?