A hydrolysis reaction in which substrate is converted to products is catalyzed by an enzyme. the reaction was determined to have a Kcat of 10000 s^-1 and a standard free energy change of -13.8 kJ/mol. answer the following questions about experiments performed with 2 nM enzyme.
a) calculate the change in free energy for this reaction at 25 degrees C under the following conditions, and indicate whether the forward reaction is favored or not.
i) [S] = 2 microM and [P]=2nM ii) [S]=2microM and [P]=2microM iii) [S]=2nM and [P]=2microM
b) if [S]=2microM, at what product concentration will the reaction be at equilibrium?
c)calculate the maximum velocity of the reaction
d)calculate the activation energy for the rate-limiting step of this reaction
e)the rate enhancement for this reaction in the presence of enzyme is 10^8. calculate the activation energy for the uncatalyzed reaction.