Through genetic engineering, a geneticist mutates the gene that encodes TBP in cultured human cells. What is the function of TBP in transcription? Predict the effect of this mutation on cells that possess it. There are two types of transcription factors; general and specific. TBP belongs to which group and what is the significance of that group?
-The following diagram represents a DNA molecule that is undergoing replication. Draw in the strands of newly synthesized DNA and identify the following items;
A) Polarity of newly synthesized strands
B) Leading and lagging strands (on both right and left half of the bubble)
C) Okazaki fragments (2 per lagging strands)
D) RNA primers (on both leading and lagging strands)
-You have just created the world's first genomic library from an African giraffe. You wish to isolate the gene encoding for muscle protein, myosin, from the genomic library of the giraffe. No gene from this animal has been previously isolated as well as amino acid sequences are available in the data base. Using this information, what two strategies can you use to isolate the giraffe's gene from its genomic library?