A bioengineer preparing cells for a cloning experiment, Chemistry

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A bioengineer preparing cells for a cloning experiment

A bioengineer preparing cells for a cloning experiment bathes a small piece of rat epithelial tissue in a TRIS buffer, (HOCH2)3CNH2. The buffer is made by dissolving 43.0 g TRIS (pKb = 5.91) in enough 0.095 M HCl to make 1.00 L of solution. What is the molarity of TRIS?

What is the pH of the buffer?

The correct answer is 0.260M and 8.53. But I don't know how to get it.

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Chemistry: A bioengineer preparing cells for a cloning experiment

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Chemistry: A bioengineer preparing cells for a cloning experiment

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