Problem - A biochemist studying the properties of a newly isolated metabolic enzyme obtains the following rate data during kinetic experiments in the absence and presence of an inhibitor. Use the Lineweaver-Burke plot and Excel.
[S] (mM) vo (without inhibitor) vo (with inhibitor)
0.10 0.45 0.21
0.12 0.54 0.25
0.17 0.67 0.32
0.25 0.87 0.45
0.50 1.25 0.74
(a) Determine the Km and Vmax for this enzyme.
(b) What type of inhibition is observed?