1) Which one is a protease inhibitor ?
a) DDT b) Thiourea c) PMSF d) SDS
2) A non specific protease is ?
a) Pronase b) Trypsin c) Chymotrypsin d) Glu-C
3) The Tandem mass analyzer not used in ESI source is ?
a) Triple quadrupole b) Ion trap c) Fourier Transform Ion Cyclotron Resonance MS d) TOF
4) Which is not a physical Map?
a) Chromosomal map b) Radiation Hybrid map c) Linkage map d) Sequence map
5) Gene finding or prediction is an important application of ?
a) Functional genomics b) Structural genomics c) Comparative genomics d) Proteomics
6) Sanger's dideoxy nucleotide method is also called ?
a) Maxam gilbert method b)SAGE c)Dye terminator sequencing d)Chain termination method
7) Microarray technology evolved from ?
a) Western blotting b) Southern blotting c) Northern blotting d) SDS - PAGE ?
8) Whole genome shotgun sequencing was done in ?
a) Institute for Genomic Research (TIGR)
b) European Bioinformatics Institute (EBI)
c) DOE-Joint Genome Institute
d) National Center for Biotechnology Information (NCBI)
9) The initiation of Human genome project took place in ?
a) 1990 b) 1980 c) 1947 d) 1981
10) 2',3' - dideoxynucleotide triphosphates (ddNTPs ) molecules differ from deoxynucleotides by having a H atom attached at which position to the carbon rather than an OH group ?
a) 2' b) 2', 3' c) 3' d) None
11) The program or algorithm that can be used for peptide mass fingerprinting is ?
a) MS-tag
b) Sequest
c) Mascot
d) SALSA
12) The Microarray technique also called Capture array ?
a) Reverse Phase protein Microarray
b) Functional protein Microarray
c) Analytical Microarray
d) DNA Microarray
13) The BAC to BAC method for sequencing the human genome is a ?
a) Map based method
b) Word method
c) Distance based method
d) Fragment based method
14) Tumor classification is done by which type of Microarray type ?
a) Expression analysis
b) Protein microarray
c) DNA microarray
d) Comparative Genome Hybridization ( CGH )
15) All the steps are required for in-gel digestion except ?
a) Reduction and Alkylation
b) Destaining
c) Proteolytic cleavage
d) Ionization
16) Tandem Affinity Purification requires fusion of which tag to the target protein of interest ?
a) His tag
b) Streptavidin tag
c) Myc tag
d) TAP tag
17) Cyanine dyes that are commonly used fluorophores with microarray are Cy ?
a) 3 , 5 b) 2 , 3 c) 5 , 6 4) 5 , 2
18) The 20 KDa protein with 20 protons in a solution will have a m/z ratio of ?
a) 1001 b) 1002 c) 40 d) 20
19) Which method is used for analysis of gene expression pattern ?
a) Microarray
b) SAGE
c) Northern Blotting
d) Maxam Gilbert method
20) The methods used by molecular biologists to produce snapshot of m-RNA in a sample of interest in the form of small tags is ?
a) PAGE
b) Reverse Transcription
c) Microarray
d) SAGE
1) Answer c) PMSF
Phenylmethyl Sulfonyl Fluoride is a serine protease inhibitor that prevents protein degradation.
2) Answer a) Pronase
Pronase , Subtilysin , Proteinase K , Pepsin are non specific protease and Trypsin ,Chymotrypsin , Glu-c , Lys C , Asp N are specific proteases.
3) Answer d) TOF (Time Of Flight )
4) Answer c) Linkage map
Linkage map is a genetic map which is a representation of genes on a chromosome arrayed in linear order with distances between loci expressed as percent recombination.
Chromosomal map , Rdiation map and Sequence map are three types of physical maps.
5) Answer c) Comparative genomics
6) Answer d) Chain termination method
7) Answer b) Southern blotting
8) Answer a) Institute for genomic research ( TIGR )
9) Answer a) 1990
10) Answer c) 3'
11) Answer c) Mascot
Mascot program uses the probability based MOWSE algorithm for identification of proteins from ESI Tandem MS Data. MS-Tag program was originally developed for analysis of PSD spectra obtained in MALDI-TOF analyses of peptides. Sequest is the first algorithm to identify proteins by matching MS-MS data to database sequences. SALSA is an algorithm for mining specific features of Tandem MS Data.
12) Answer c) Analytical microarray
13) Answer a) Map based method
14) Answer d) Comparative Genome Hybridization
15) Answer d) Ionization
Four steps used in in-gel digestion are
1) Destaining
2) Reduction
3) Alkylation
4) Proteolytic cleavage.
16) Answer d) TAP -tag
17) Answer a) 3 , 5
18) Answer a) 1001
The absolute mass of a singly protonated 20 KDa protein (m/z) is 20,001. The 20 KDa protein however in a solution accepts 10-30 protons.
The m/z ratio of this protein in a solution will then be 20,020/20 = 1001.
19) Answer b) SAGE
20) Answer d) SAGE