1. In a common protein analysis, a dye binds to the protein and the color of the dye changes from brown to blue. The intensity of the blue color is proportional to the amount of protein present.
Protein (µg): 0.00(blank) 9.36 18.72 28.08 37.44
Absorbance: 0.466 0.676 0.883 1.086 1.280
(a) 10 mL of an unknown sample was placed in a 100 mL volumetric flask and diluted to the mark with the appropriate solvent. This solution gave an absorbance reading of 0.973. Calculate the number of micrograms of protein in the original unknown sample