--%>
Assignment Help - homework help

Explanation of double immuno diffusion method

Give a brief explanation of double immuno diffusion method.

E

Expert

Verified

In this technique, both antibodies and antigens diffuse radically from wells towards each other by establishing a concentration gradient. As correspondence is reached, a perceptible line of the precipitation is observed.

The patterns of the precipitin lines which are formed when two dissimilar antigens are placed in adjacent wells designate whether they share any general epitope or not.

Identity takes place when two antigens share identical epitopes; therefore, the line of precipitation which is formed through them will fuse to give single curve line of the identity.

Non-identity takes place when two antigens are not linked. The antiserum form independent precipitin lines which is cross each other.

Partial identity takes place when two antigens share common epitope. The antiserum forms line of characteristics with common epitope and a curved spur with unique epitope.

   Related Questions in Biology

  • Q : Meaning of Trachea What do you mean by

    What do you mean by the term Trachea?

    		•
  • Q : Pathway of degradation of the organic

    Why the Krebs cycle is also termed as the final common pathway of degradation of the organic compounds?

    		•
  • Q : Hematopoiesis Explain where does

    Explain where does hematopoiesis occur?

    		•
  • Q : What is Benchmarking Benchmarking : It

    Benchmarking: It is a measurement of the quality of an organization's policies, programs, products, strategies, and so on, and their comparison with the standard measurements, or alike measurements of its peers.

    Q : Define Wide Area Network or WAN Wide

    Wide Area Network (WAN): The Data and voice communication network which extends beyond the geographical limitations of a local area network (abbreviated as LAN). In a usual configuration, a WAN consists of numerous LANs at dispersed p

    		•
  • Q : Tissue in heart Specify the tissue

    Specify the tissue which the heart is made up? And also explain how this tissue is oxygenated and nutrified?

    		•
  • Q : Explain Homogenization Homogenization :

    Homogenization: It is intensive blending of mutually associated substances or groups of mutually associated substances to form a constant of distinct insoluble phases (sometimes with the addition of surfactants) to receive an emulsion or suspension.

    		•
  • Q : Define Leadership Leadership :

    Leadership: Leadership is a critical role in any organization. It comprises stating the direction of a team and communicating it to the people, motivating, inspiring and em-powering them to contribute to attaining organizational success. The leadershi

    		•
  • Q : Physiology The study of different types

    The study of different types of body functions or processes like respiration, growth and photosynthesis is called physiology. Physiology is the science of the function of living systems. Physiology includes how organisms, organ systems, cells, organs or biomolecules c

    		•
  • Q : Tolerance in Homo neanderthalensis Homo

    Homo neanderthalensis was an extinct human species that lived in Europe and western Asia, and members of this species subsisted exclusively on hunted and gathered foods. Based on your knowledge of milk tolerance in humans, would you suppose that Homo neanderthalensis

    		•