Problem: In our chromatography lab, the anion exchanger DEAE has a pka of 10. If your professor screwed up and prepared the DEAE at a pH of 10.4 (instead of 7), what would be the predicted percent yield loss of alkaline phosphatase from what went into the column, assuming all the AP was completely negatively charged and the nothing else in the experiment contributed to a loss in yield (show your work)? At this incorrect pH, would we run the risk that the DEAE would become a cation exchanger? Why or why not?