1. HCL is a significant component of gastric juice. what is the concentration of chloride ion in gastric juice at pH=1.27?
2. Tris is widely used in a biochemical laboratory. Its acid dissociation constant is 8.32 x 10^-9. You have available at your lab bench a 0.1 M solution of Tris in its protonated form, 0.1 M solution of HCL and NaOH, and ample distilled water. Describe the preparation of 1L solution of 0.02M Tris buffer, PH 7.8.
3. What volume of glacial acetic acid (d= 1.06g/ml) and what weight of solid potassium acetate are required to prepare 5 liters of 0.2M acetate buffer,PH 5.0?