a. We need 20 micrograms of the protein for an experiment. What volume of protein solution do we need?
b. Suppose we want a soln containing 150 micrograms of the protein at a concentration of .5 mg/ml. To do this we will first evaporate the liquid from enough of the protein soln to get 150 micrograms. How much coln do we need to start with? How much H2O do we add to get the desired concentration?
c. If the protein has a MW of 15000, express its initial concentration in micromoles/ml and in micromoles/microliters. If we want 2 micromoles of the protein for a rxn, how much of the original soln do we need?
d. Suppose we want 1 ml of a 10 microgram/ml soln. How much H2O and protein stock must we add to get this?
e. Suppose we want 100 microliters of .1 micrograms/ml soln. How much H2O and protein stock must we add to get this?