Suppose that you carry out an analytical procedure to generate a linear calibration curve like that shown in the figure below. Then you analyze an unknown and find an absorbance that gives a negative concentration for the analyte Select the true statements. (Select all that apply.)
1.If the negative value is within experimental error, no detectable analyte is present.
2.If the negative concentration is beyond experimental error, there is something wrong with your analysis, and the same is true for a value above 100% of the theoretical maximum concentration of an analyte.
3.Hopefully, the negative value is within experimental error of 0.
4.It is impossible to explain why you get values below 0 or above 100%.
5.It is possible to get values below 0 or above 100% is if you extrapolated the calibration curve past the range covered by standards, and the curve is not linear.