The Z factor (aka Z') in Zuck et al, 2004, was calculated to be what?
According to Z-factor formula, smaller standard deviations of positive and negative controls AND larger differences in mean values of positive and negative controls will result in larger Z values.
A. True
B. False
In the final protocol for the high throughput screen of Zuck, et al. (2004), which of the following were steps taken to improve thereproducibilityof the high throughput assay?
A. Only two batches of cells were prepared for the entire screen of 270,000 compounds
B. A high (>10x) noise level was achieved in developing the screen.
C. Compounds were reviewed by medicinal chemists to eliminate undesirable compounds.
D. Clavulanic acid was included in the protocol to minimize the signal.
A high throughput screen based on a FRET assay is being planned, and project team members are discussing the compound concentration that will be used to screen the diversity library. Which of the following would motivate the team to use a higher-than-normal concentration of compound in the screen? (Please refer to Walters and Namchuk paper, Figure 1)
A. In the past, many of the compounds in the library have been found to have intrinsic fluorescence.
B. In the past, many of the compounds in the library have been found to have "drug-like" properties, based on the "Rule of Five"
C. In the past, many of the compounds in the library have been found to be insoluble.
D. In the past, similar screens have yielded a low number of hits