Assignment:
A mixture of organic acids was separated by reversed-phase liquid chromatography. The data obtained for the chart recording were as follows: For acid A, the peak appeared 22 cm after injection with a full width at baseline of 8 cm. For acid B, the peak appeared 34 cm after injection with a full width at baseline of 6 cm. A non-retained compound generated a peak that appeared 4 cm after injection. The mobile phase flow rate in the column was 20 mL/min. The recorder chart speed was 5 cm per minute. Last, the column was 32 cm in length. Calculate:
a. The adjusted retention time for peak A
b. The capacity factor for peak A
c. The resolution of compounds A and B.
d. The number of theoretical plates in the column for acid B.
e. The height equivalent to a theoretical plate for acid B
Provide complete and step by step solution for the question and show calculations and use formulas.