Protein Sequencing Question. Determine the sequence of a peptide consisting of 14 amino acids on the basis of the following data:
Amino acid composition: (4S, 2L, F, G, I, K, M, T, W, Y)
N-terminal analysis of full peptide: S
Carboxypeptidase digestion: L
Trypsin digestion: (peptide composed of 3S, 2L, F, I, M, T, W) and another composed of (G, K, S, Y)
Chymotrypsin digestion: (one peptide composed of (F, I, S), another of (G, K, L), another of (L, S), another of (M, T), another of (S, W) and another of (S, Y)
N terminal analysis of the (F, I, S) peptide is S
Cyanogen bromide treatment of the full peptide results in one peptide composed of (2S, F, G, I, K, L, M*, T, Y) and another composed of (2S, L, W)
M*, methionine detection as homoserine