Problem:
In a Flow Cytrometry, one can add an Isotype Control Antibody to allow another antibody to bind more specific to the cells.
Question: how can the Isotype Control Antibody add specificity to the other antibody?
My thought was, that the Isotype Control Antibody binds to all the antibody receptors, but with a "weak" binding. When the other antibody (e.g. CD73) tries to bind, it can make a stronger binding with the CD73 antibody receptor, kicking the Isotype Control Antibody off it.
However i'm entirely not sure this is correct... Could anyone explain how the Isotype Control Antibody helps the other antibody (eg.g CD 73) to bind more specific? Describe your answer.