How can calculate the purpose of using the brine
How can calculate the purpose of using the brine in the experiment, in a lab we completed a reaction, and then washed the ether layer with brine.
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The protein has a MW of 35,000. One is interested in determining the affinity of P for leonine. An amino acid binding protein (P) is released from E. coli by the osmotic shock, and purified to homogeneity.
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