An SDSU junior has grown a bacteriophage stock during a BIOL 350, General Microbiology, lab. He determines it has a concentration of 7.5 x 1014 phage/mL. He is given a Salmonella typhimurium host culture with a concentration of 8.1 x 1012 cells/mL and adds a 300 uL aliquot of his bacteriophage stock to 5 mL of Salmonella cells.
a. What is the m.o.i. in phage/cell? Answer to one decimal place.
b. What is the total volume of his phage + host cell mixture? Answer to one decimal place.
c. What volume, in microliters, of bacteriophage stock should he add to 3 mL of host cells to have a 0.7 moi? Answer to the nearest whole number.