Assignment:
Finding percentage of protein
1. Subcellular fractionation of rat liver resulted in the following samples:
Fraction speed (xg) Volume(ml) Protein (mg/ml)
P-1 2800 10 6
P-2 12000 10 4
P-3 27000 8 4
P-4 75000 5 2.5
S non-sedimentable 25 6.5
2. Analysis of the frations resulted in the following enzyme activities expressed as nmoles/min/ml of sample fraction.
Fraction Citrate synthase
P-1 0.20
P-2 8.00
P-3 6.00
P-4 0.50
S 0.04
Question: Find the % protein for each fraction.