Explain the free amine and concentrated hcl


A. Explain what volume of concentrated HCl and what weight of tris (hydroxymethyl) amino methane (as the base) are needed to make the 100 ml of 0.25M buffer, pH 8.0? [pKa of trishydrochloride is 8.0]

B. Explain how to prepare 250ml of 0.6M triethanolamine buffer, pH7.2, from the free amine and concentrated HCl ?(pKa for the amine hydrochloride is 7.8)

 

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Chemistry: Explain the free amine and concentrated hcl
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