Problem:
The hydrolysis of glucose-6-phosphate to glucose and phosphate is an important step in glycogen catabolism. The free glucose formed by this reaction is released intto the blood for transport to cells in need of energy
glucose-6-phosphate +H20----> Glucose/phosphate
\Delta G{}'^{\circ} for this reaction is -3.3 kcal/mol at 25 degrees and pH 7.0
Required:
Question 1: Calculate \Delta G{}'^{\circ} at 25 degrees when the conventration of glucose-6-phosphate is 20 \muM and the glucose and phosphate conventrations are 5mM each. Assume the appropriate enzyme is presnet to catalyze this reaction
Question 2: In which direction would you expect the reaction to proceed under the conditions defined in question 1?
Show the pedigree and your calculation.