Explain how to make solution of phosphate buffered saline


Problem:

To make up a solution of phosphate buffered saline (PBS), you need 15 mM Na2HPO4 (anhydrous) (FW: 141.96 g/mol), 0.25 M NaCl (FW: 58.44 g/mol), and 2 mM KH2PO4 (FW: 136.09 g/mol).

Required:

Question: How many grams of each will you need to make up 750mL of PBS?

Show your calculations, but you do not need to write out any explanation.

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Biology: Explain how to make solution of phosphate buffered saline
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