Does substrate binding achieve equilibrium or steady state


For a MM reaction
k1=5x10^7
k-1=2X10^4
k2=4x10^2

i calculated Km and Ks to be equal at about 4 x10^-4M

Does substrate binding achieve equilibrium or the steady state?

Also, you helped me with my table of amino acids....could you clarify when you have ph=pkA how you get whether it is -1/2 or +1/2 or in the other case +1/3 or -1/3......how do you tell whether it is + or -
you also misadded the charges on the last AA for pH 6 just FYI :)

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Biology: Does substrate binding achieve equilibrium or steady state
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