Problem:
When performing a DNA cloning, sometimes PCR amplicon is run in agarose and it is detected by ethidium bromide marking under UV light. After that, gel is sliced, DNA extracted from gel....... until eventually you have your transgenic organism. The thing is that when doing so, you use the very same DNA sample that was "contaminated" by ethidium bromide,
Required:
Question: Does this DNA you are using for transformation contain ethidium bromide?
Question: Iif so, how can it affect in numbers to mutation risk?
Give reasoning with your answer.