At 7.6 pH an enzyme catalyzed reaction was carried out in a 0.22M Tris buffer. The pKa of Tris is 8.1. As a result of this reaction, 0.03 moles/liter of H+ was formed.
1. At the beginning of the reaction explain the ratio of Tris conjugate acid to Tris conjugate base?
2. At the beginning of the reaction determine the concentrations of the Tris conjugate acid and base?
3. Explain the reaction by which the buffer maintained near the constant pH
4. Determine the concentrations of Tris conjugate acid and the base at the end of the reaction?
5. Determine the pH at the end of the reaction?